Mapping structure, function, and behavior in live C. elegans embryos.
In this talk, I will describe our group's efforts to map brain formation in live worm (the nematode C. elegans) embryos. Our goal is to follow the development of all 222 embryonic neurons as they form the functional brain. I will attempt to introduce the audience to the techniques involved, including advanced light microscopy and image processing. Applications of these technologies will be presented, including computational methods for untwisting worm embryos and calcium imaging in freely moving embryos. Finally, I will describe efforts to quantitatively map the 'behaviorome' of freely moving embryos using microscopy.